EPA METHOD 533
US EPA Method 533 employs solid-phase extraction (SPE) liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the precise determination of 25 specific PFAS in drinking water. Enhancing selectivity through Multiple Reaction Monitoring (MRM), the method utilizes isotope dilution for quantification. Addressing some of the more challenging C4 and C5 acids and sulfonates present in EPA methods 537 and 537.1, Method 533 focuses on short-chain PFAS analysis by limiting chain length to C12 acids. Additionally, it introduces certain emerging PFAS using weak anion exchange (WAX) SPE.
technical data sheet
METHOD CODE
LAB-TM-080
EQUIPMENT
Ultra-Performance Liquid Chromatography /
Tandem Mass Spectrometry (UPLC-MS/MS)
Tandem Mass Spectrometry (UPLC-MS/MS)
METHOD REFERENCE
EPA Method 533
REPORTABLE UNIT
ng/L
SAMPLE SIZE REQUIREMENTS
3x 250 mL
AVAILABLE FOR
Drinking Water
ANALYTE LIST
XXX
ISTD RELATIONSHIP
TARGET ANALYTES
CASRN
INTERNAL STANDARD (ISTD) ANALYTES
XXX
TARGETS
ISTDs
PFBA
375-22-4
M4-PFBA
XXX
PFBA
M4-PFBA
PFPeA
2706-90-3
M5-PFPeA
XXX
PFPeA
M5-PFPeA
PFHxA
307-24-4
M5-PFHxA
XXX
PFHxA
M5-PFHxA
PFHpA
375-85-9
M4-PFHpA
XXX
PFHpA
M4-PFHpA
PFOA
335-67-1
M8-PFOA
XXX
PFOA
M8-PFOA
PFNA
375-95-1
M9-PFNA
XXX
PFNA
M9-PFNA
PFDA
335-76-2
M6-PFDA
XXX
PFDA
M6-PFDA
PFUnDA
2058-94-8
M7-PFUnDA
XXX
PFUnDA
M7-PFUnDA
PFDoDA
307-55-1
M2-PFDoDA
XXX
PFDoDA
P2-PFDoDA
PFBS
375-73-5
M3-PFBS
XXX
PFBS
M3-PFBS
PFPeS
2706-91-4
M3-PFHxS
XXX
PFPeS
M3-PFHxS
PFHxS
355-46-4
M8-PFOS
XXX
PFHxS
M3-PFHxS
PFHpS
375-92-8
M2-4:2 FTS
XXX
PFHpS
M8-PFOS
PFOS
1763-23-1
M2-6:2 FTS
XXX
PFOS
M8-PFOS
4:2 FTS
757124-72-4
M2-8:2 FTS
XXX
4:2 FTS
M2-4:2 FTS
6:2 FTS
27619-97-2
M3-HFPO-DA
XXX
6:2 FTS
M2-6:2 FTS
8:2 FTS
39108-34-4
XXX
XXX
8:2 FTS
M2-8:2 FTS
HFPO-DA
13252-13-6
XXX
XXX
HFPO-DA
M3-HFPO-DA
ADONA
919005-14-4
XXX
XXX
ADONA
M4-PFHpA
PFMPA
377-73-1
XXX
XXX
PFMPA
M4-PFBA
PFMBA
863090-89-5
XXX
XXX
PFMBA
M5-PFPeA
NFDHA
151772-58-6
XXX
XXX
NFDHA
M5-PFHxA
9Cl-PF3ONS
756426-58-1
XXX
XXX
9Cl-PF3ONS
M8-PFOS
11Cl-PF3OUdS
763051-92-9
XXX
XXX
11Cl-PF3OUdS
M8-PFOS
PFEESA
113507-82-7
XXX
XXX
PFEESA
M3-PFBS
