EPA METHOD 533
US EPA Method 533 employs solid-phase extraction (SPE) liquid chromatography/tandem mass spectrometry (LC/MS/MS) for the precise determination of 25 specific PFAS in drinking water. Enhancing selectivity through Multiple Reaction Monitoring (MRM), the method utilizes isotope dilution for quantification. Addressing some of the more challenging C4 and C5 acids and sulfonates present in EPA methods 537 and 537.1, Method 533 focuses on short-chain PFAS analysis by limiting chain length to C12 acids. Additionally, it introduces certain emerging PFAS using weak anion exchange (WAX) SPE.
technical data sheet
METHOD CODE
LAB-TM-080
EQUIPMENT
Ultra-Performance Liquid Chromatography /
Tandem Mass Spectrometry (UPLC-MS/MS)
Tandem Mass Spectrometry (UPLC-MS/MS)
METHOD REFERENCE
EPA Method 533
REPORTABLE UNIT
ng/L
SAMPLE SIZE REQUIREMENTS
3x 250 mL
AVAILABLE FOR
Drinking Water
ANALYTE LIST
TARGET ANALYTES
CASRN
INTERNAL STANDARD (ISTF) ANALYTES
PFBA
375-22-4
M4-PFBA
PFPeA
2706-90-3
M5-PFPeA
PFHxA
307-24-4
M5-PFHxA
PFHpA
375-85-9
M4-PFHpA
PFOA
335-67-1
M8-PFOA
PFNA
375-95-1
M9-PFNA
PFDA
335-76-2
M6-PFDA
PFUnDA
2058-94-8
M7-PFUnDA
PFDoDA
307-55-1
M2-PFDoDA
PFBS
375-73-5
M3-PFBS
PFPeS
2706-91-4
M3-PFHxS
PFHxS
355-46-4
M8-PFOS
PFHpS
375-92-8
M2-4:2 FTS
PFOS
1763-23-1
M2-6:2 FTS
4:2 FTS
757124-72-4
M2-8:2 FTS
6:2 FTS
27619-97-2
M3-HFPO-DA
8:2 FTS
39108-34-4
XXX
HFPO-DA
13252-13-6
XXX
ADONA
919005-14-4
XXX
PFMPA
377-73-1
XXX
PFMBA
863090-89-5
XXX
NFDHA
151772-58-6
XXX
9Cl-PF3ONS
756426-58-1
XXX
11Cl-PF3OUdS
763051-92-9
XXX
PFEESA
113507-82-7
XXX
